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The soil is alive!

Jodi DeJong-Hughes

Introduction

In most ecosystems, more life and diversity exists underground than above. The soil is home to a vast array of organisms, including bacteria, cyanobacteria, algae, protozoa, fungi, nematodes, and mites, insects of all sizes, worms, small mammals and plant roots.

Soil organisms play critical roles in plant health and water dynamics. Some of the processes soil organisms contribute to are nutrient cycling, nutrient retention, water infiltration and water-holding capacity, disease suppression, and degradation of pollutants. They also increase the biological diversity of the soil and improve soil structure.

Soil biological processes are responsible for supplying approximately 75 percent of the plant available nitrogen and 65 percent of the available phosphorus in the soil. Like all organisms, those inhabiting soil need food and a favorable environment. Adequate organic matter content, ample aeration, moderate moisture, neutral pH, and warm temperatures all favor increased microbial activity.

By maintaining a high soil organic matter content, food and a favorable habitat can be built for a diverse community of soil organisms. Not only does organic matter provide good habitat, but it also is of great benefit to chemical and physical soil characteristics. Moisture, pH, nutrient supply, and the biological community are all more stable, or "buffered," as soil organic matter increases. Organic material also helps to maintain soil porosity, which is essential because most beneficial soil microbes and processes are aerobic, requiring oxygen.

The root zone

Most biological activity takes place in the top 8-12 inches in the soil profile. The rhizosphere, or rooting zone, is an area of intense microbial activity and is integral to plant and soil relationships.

Plant roots leak energy-rich carbon compounds, sugars, amino and organic acids called exudates. Every plant species leaks a unique signature of compounds from their roots. Different microbes are attracted to different chemical exudates. The plants grown play a large role in determining the microbial community in the soil below.

Bacteria

actinomycetes

Photo courtesy of Soil Biology Primer

Photo 1. Actinomycetes

Bacteria are the smallest and most numerous of organisms in the soil. Collectively, there are billions of individuals in an ounce of soil. Some experts think that less than half of the species of bacteria, and therefore their functions, have been identified.

Most bacterial species are decomposers that live on simple carbon compounds, root exudates and plant litter. They are the first on the scene when nutrients and residue are added to the soil. They convert these compounds into forms readily available to the rest of the organisms in the food web. Actinomycetes are an example of microbial decomposers (Photo 1). They grow hyphae like fungi, but are closer to bacteria in their evolutionary history. Actinomycetes arrive later in the decomposition process, and are responsible for the "earthy" smell of freshly tilled soil.

Rhizobium

rhizobium-bacteria

Photo: Stephen Temple

Photo 2. Rhizobium bacteria nodules on soybean roots.

Other bacterial species form partnerships with plants. The most well-known of these are the nitrogen (N) fixing bacteria, rhizobium, that form symbiotic relationships with legumes, such as alfalfa, soybeans, edible beans, and clover. Rhizobium infect the roots of the host plant (Photo 2) and convert atmospheric nitrogen (N2) into plant-available ammonium (NH4+). In return for supplying the plant with all of its nitrogen needs, the host plant supplies the rhizobium with simple carbohydrates. The plant can give up to 20 percent of its supply of carbohydrates to the bacteria. However, if there is a sufficient supply of nitrogen in the soil to meet the plant's needs, the plant will not engage in a relationship with the bacteria.

A healthy, well-nodulated legume crop can supply its own N needs. In addition, it can produce extra N which will be available for the next crop. This results in a "nitrogen credit," which should be factored in when making N fertilizer recommendations. It may be difficult to account for all of the sources that contribute to this credit. One source is nitrogen mineralized from the old roots and leaves of the legume. Another source is the beneficial microbial community created by the legume. Table 1 shows the nitrogen credits that can be expected from different legumes.

Table 1. Nitrogen credits from different legume species in the first year of corn following a legume (10).

Legume crop 1st year nitrogen credit
pounds N per acre
Soybeans 40
Alfalfa (terminated)
4 or more plants/ft2 150
2-3 plants/ft2 100
1 or less plants/ft2 40
Group 1 crops1 75
1 Group 1 crops include alsike clover, birdsfoot trefoil, grass/legume hay, grass legume pasture, fallow, and red clover.

For each legume species there is a corresponding rhizobium. For example, the species of rhizobium for soybeans is Bradyrhizobium japonicum while the species for alfalfa is Sinorhizobium meliloti. To effectively inoculate the soil, the rhizobium specific to the legume grown must be used. When a legume is introduced into a soil that has not been previously cropped to that species, it is unlikely that the soil will contain sufficient numbers of the correct rhizobia. In these cases, a yield response to seed inoculation is likely. Where such inoculation is properly carried out, senescence of the nodules at the end of the growing season will return large numbers of rhizobia to the soil. This should ensure that inoculation in future years will be unnecessary. However, there have been benefits from inoculation if the crop has not been grown in the last three to five years in that field.

Rhizobium are easily applied to the seed and the preferred inoculant for any legume is a sterile-peat based culture. However, inoculants may also be supplied in a non-sterile peat, as a liquid or frozen concentrate, or as a clay-based or granulated peat preparation.

In the US, more than one strain is generally included in an inoculant, but in Australia, Canada and France, the inoculant usually contains only a single strain. Control of inoculant quality is also more formalized in Australia and Canada than in the U.S. See more information on Rhizobium inoculants.

Nitrifying bacteria

Nitrifying bacteria, Nitrosomonas and Nitrobacter, convert ammonium (NH4+) to nitrite (NO2-) and then to nitrate (NO3-). Nitrate is the preferred form of nitrogen for row crops.

However, nitrate is also the form of nitrogen that is most easily leached out of the root zone.

Fall applications of anhydrous ammonia should be made only after the soil temperature at a 6-inch depth is 50°F or below. Nitrifying bacteria have very low activity below this temperature, so the risk of over-winter nitrate leaching losses are reduced. In addition, the very low vapor pressure of ammonia below 50°F virtually eliminates direct loss of ammonia during or after application. Table 2 lists the time it takes nitrifying bacteria to convert different N fertilizers to nitrate-N.

Table 2. Rate of conversion of different fertilizer sources to nitrate-N (7).

Fertilizer material Conversion time to nitrate
Ammonium Sulfate
10-34-0, MAP, DAP
1-2 weeks
Anhydrous Ammonia 3-8 weeks
Urea 1½ - 3 weeks
Ammonium Nitrate 50% is nitrate: 0 weeks
50% is ammonium: 1-2 weeks
UAN 50% is urea: 1½ - 3 weeks
25% is ammonium: 1-2 weeks
25% is nitrate: 0 weeks

Denitrifying Bacteria

Some bacteria can live without oxygen or in anaerobic conditions. Denitrifying bacteria are an example of anaerobic bacteria that convert nitrate (NO3-) to nitrous oxide gas (N2O) and then to nitrogen gas (N2). Once the nitrogen is in a gaseous form, it is no longer available for plant uptake and will escape back to the atmosphere. There are 13 different species of bacteria responsible for this process. They become active when the soil has at least 50 percent of its pore space filled with water and are most active when the soil is saturated. Denitrifying bacteria under saturated conditions can convert 2 to 4 pounds of nitrate to a gaseous form per acre per day. There are commercial products available to reduce the potential for nitrogen loss from either volatilization or leaching. Below, the two most popular choices are described.

N-Serve

Photo: Mary Ann Bruns, Center for Microbial Ecology, Michigan State University

Photo 3. Cross-section of nitrifying bacteria.

The goal for all fall applications of N fertilizer should be to keep as much nitrogen as possible in the ammonium (NH4+) form going into the winter. With this strategy, only small amounts are present in the nitrate (NO3-) form and, thus, not subject to spring losses caused by leaching and denitrification.

Nitrapyrin, known commercially as N-Serve, selectively inhibits Nitrosomonas bacteria (Photo 3), which in turn slows or stops the conversion of ammonium to nitrite. N-Serve is typically added to anhydrous ammonia and liquid fertilizers to prevent the conversion of ammonium to nitrate.

An Illinois study found that the earlier ammonia is applied in the fall, the more ammonia is converted to nitrate, increasing the potential for leaching (Table 3). Adding a nitrification inhibitor decreased the conversion rate but did not stop it entirely.

Table 3. Rate of conversion of anhydrous ammonia to nitrate by May 25 by application date with and without a nitrification inhibitor in northern Illinois, 2004. Source: Robert Hoeft, University of Illinois.

Application date Ammonia without N inhibitor Ammonia with N inhibitor
percent conversion to nitrate (%)
November 1, 2003 85 55
December 1, 2003 60 45
March 15, 2004 50 20
April 1, 2004 35 15

Another study was conducted at Waseca, MN to look at the effects of a nitrification inhibitor on corn yield over a 14-year period. Average corn yields showed that yield from spring-applied anhydrous ammonia by itself and fall-applied anhydrous ammonia with N-Serve was equal. However, the yield from fall applied nitrogen without N-Serve was lower for only 6 out of the 14 years. Therefore, there is no guarantee that use of N-Serve with anhydrous ammonia (82-0-0) in the fall will increase yields. Loss of nitrogen is a complex relationship and not an exact science. It is based on the timing of nitrogen application, soil temperatures, rainfall totals and intensity, and soil texture. Both of these studies stress that the use of N-Serve must be viewed as an insurance policy and not a guarantee.

Agrotain

Agrotain is a urease enzyme inhibitor that is designed to delay ammonia volatilization when applied with urea ammonium nitrate (UAN) or urea. This delay allows more time to get the urea incorporated into the soil through rainfall before N losses occur. Agrotain has the greatest benefit in reducing volatilization when urea or UAN is surface-applied or where limited or no soil incorporation occurs, such as in a no-till system. Varsa et al. (14), found that in most studies Agrotain in combination with urea gave greater yield increases (average 14 bu/ac) than did Agrotain used with UAN (average of 7 bu/ac). The greatest yield benefit from Agrotain was observed when applied with urea to corn in a corn-soybean rotation (30 bu/ac). Yield decreases with application of Agrotain also occurred; yield reductions of 10 bu/a or more were seen in 7 percent of the sites. Consistent crop yield increases are not expected every year or on all fields. Benefits will likely occur 30 to 40 percent of the time; with negative impacts on yield 5 to 10 percent of the time. Overall, these data highlight that when conditions for N loss exist, Agrotain can help prevent N loss. However, yield gains will not necessarily be realized every year (7).

Fungi

fungal-hyphae

Photo: Gupta Vadakattu, CSIRO group

Photo 4. Fungal hyphae binding soil particles together into aggregates.

white-mold-soybean

Photo: X.B. Yang, University of Minnesota

Photo 5. White mold infection on soybean.

Fungi are a critical contributor to decomposition and nutrient cycling. Fungal activity is often slower to develop than bacteria and is more active on relatively high carbon to nitrogen ratio (C:N) and lignin-containing materials, such as corn residue. Fungi, more so than bacteria, enhance soil physical structure and add chemical compounds that bind soil aggregates and serve as building blocks for organic matter (Photo 4).

Although most fungi are beneficial, there are several species that are notorious as pathogens. These include the fungi Sclerotinia (White Mold) (Photo 5), Fusarium, Pythium, Phytophthora, and Rhizoctonia.

Arbuscular Mycorrhizae

endotrophic-mycorrhiza

Photo: Marcia Wicklow-Howard, Boise State University

Photo 6. Endotrophic mycorrhiza infecting a plant root.

One particularly beneficial and important type of fungi is Arbuscular Mycorrhizae (AM). These fungi form a mutually beneficial relationship with 80 percent of all land plants including most agricultural crops (Photo 6). AM fungi are critical in the early establishment and growth of corn and most cereal crops. They are also important to sunflower, soybeans, flax and potatoes.

Mycorrhizal hyphae are 1/10th the size of root hairs and they extend throughout the soil mobilizing nutrients for the plant (Table 4). In exchange, the fungi receive food from the plant in the form of carbohydrates. Similar to the relationship between a leguminous plant and a bacterial rhizobium, colonization of plant roots by AM will be inhibited if the plant has sufficient levels of soil phosphorus.

Table 4. Nutrients taken up by corn plants with and without AM infection when no phosphorus is applied (8).

Element No Mycorrhizae With Mycorrhizae
  Micrograms/plant
Phosphorus 750 1340
Potassium 6,000 9,700
Calcium 1,200 1,600
Magnesium 430 630
Zinc 28 95
Copper 7 14
Manganese 72 101
Iron 80 147
trapped-nematode

Photo: George Barron, University of Guelph

Photo 7. A nematode being trapped by the hyphae of fungi.

Due to the hyphal density of the fungi on the plant root, AM are known to increase resistance of the host plant to root diseases by acting as a physical barrier. Photo 7 shows the direct effect of hyphae on a nematode. Additionally, AM can increase water efficiency and drought tolerance in times of low soil moisture.

Fungi need oxygen, nutrients, neutral pH, and a host to survive. Crops that are non-hosts and therefore do not support AM establishment are canola, sugarbeet, mustard, lupines and other brassicas. Frequently, saturated soils or black fallow will dramatically decrease the number of AM. Other agents that will decrease their populations are ammonia present in alkaline soils, aluminum, and possibly tannins from leaf litter.

Mycorrhizal Inoculants

Similar to rhizobium, AM fungi inoculants often show benefits to crops when low populations of the the correct species or strains are present in the soil. Generally, inoculated organisms will not last long if the environment is not suitable. If the environment is suitable, the organisms are probably there anyway. Good organic matter content along with good moisture and aeration are all that most beneficial microbes will need.

Unlike rhizobium, AM are not easily applied to the seed. Mycorrhizae cannot be grown in artificial medium and must be cultured on plant roots. Therefore, the seed is coated with clay granules that contain the appropriate strain of AM. Because of this process, inoculation of AM is difficult and economical only for horticulture crops, turf grass, or high value crops.

Nematodes

soil-nematode

Photo 8. Soil nematode.

Nematodes are microscopic, worm-like organisms that are very abundant in the soil (Photo 8). Nematodes are beneficial because they enhance the rate of nutrient cycling by grazing on bacteria, other microorganisms, or by eating organic matter and debris. A few nematodes are plant parasites, such as the soybean cyst nematode. Root-feeding nematodes use their stylets to puncture the thick cell wall of plant root cells and siphon off the internal contents (Photo 9).

root-feeding-nematode

Photo: Elaine R. Ingham

Photo 9. Root feeding nematode.

Bacteria and fungi are high in protein that in turn is high in nitrogen. When these nematodes eat bacteria or fungi they digest the protein and excrete nitrogen into thin a form that becomes available to plants (2).

Nematodes are a biological control agent of armyworms, root weevil, black cutworm, grubs, Japanese beetles, ants, fleas, and over 250 other soil dwelling pests. When nematodes come into contact with their prey, they attack by entering through body openings or simply by boring through the body wall. Once inside, the nematode releases mutualistic bacteria from its gut that kills the host organism within 24-48 hours. Such nematodes will feed and reproduce before exiting in search of fresh prey.

Beneficial soil nematodes are usually more abundant in crop management systems that include multiple crop sequences, reduced cultivation, and the addition of organic amendments. Infective juveniles are compatible with most, but not all, agricultural chemicals under field conditions. Many chemicals recognized to be toxic to nematodes have only a transient effect, and nematodes recover quickly after exposure.

Management That Decreases Microbial Populations

Some fertilizers and agrichemicals have a negative impact on soil microbes. Anhydrous ammonia, some nematicides, and ammonia-rich and sulfur-rich fertilizers can directly harm soil life or indirectly hamper their growth by decreasing soil pH (acidification).

Increased pest and pathogen problems are often caused by insufficient rotation interval between crops. This is at least partially due to reduced biological diversity and weakened communities of beneficial organisms. If the soil microbes are not working for you they are more likely to work against you. Soil biota includes hundreds of pathogens, which are more likely to dominate the soil community if beneficial organisms have declined. When beneficials dominate the community, they suppress pathogens by competition and predation, and act as a physical protective barrier for plant roots.

Tillage directly affects soil porosity and the placement of residues. Porosity determines the amount of air and water the soil can hold. Placement of residues affects the soil surface temperatures, rate of evaporation and water content, nutrient loading, and rate of decay. In other words, tillage collapses the pores and changes the water holding, gas, and nutrient exchange capacity of the soil. Reducing soil disturbance increases the diversity and population of soil organisms. These soils release nutrients gradually and have better soil structure than full width tillage systems (2).

Management That Increases Microbial Populations

A more diverse soil community results in a more flexible soil. This means a soil has the ability to successfully grow a number of crops and is resilient in drought, low nutrient conditions and after a disturbance. Agricultural practices such as tillage, crop rotations and fertilizer inputs affect the numbers, diversity and functioning of the soil community (2).

Organic matter from roots, plant biomass, manure, and compost provide the food energy to support the biological community. Cover crops and green manure crops increase the length of time that plants are actively growing in a soil, providing a steady influx of food for soil microbial populations. Cover crops also aid in reducing soil erosion. Diverse crop rotations can also help disrupt some pathogen cycles.

To promote soil biodiversity:

It is important to remember the general philosophy that beneficial soil organisms "need to be needed." That is, if the farm system depends on and supports their activities, more biomass and positive activities will develop. If the farm system depends solely on chemical inputs instead of biological inputs, beneficial biomass and activities will decline.

Resources

  1. Carpenter-Boggs, L. 2004. Enhancing Soil Fertility in Organic and Low-Input Agriculture. Department of Plant Pathology, Washington State University.
  2. Clapperton, J. Earthspirit Land Resource Consulting, Florence, MT. Earthspiritconsulting@gmail.com. Personal communication.
  3. Clapperton, J. The real dirt on no tillage. No-Till on the Plains, Wamego, KS. Available at http://www.notill.org/KnowledgeBase/03_realdirt_Clapperton.pdf. (verified 12 November 2008).
  4. Ingham, Elaine. Soil Foodweb, Inc. Corvallis, OR. http://www.soilfoodweb.com/. (verified 12 November 2008).
  5. Johnson, J. 2004. Soil microbial communities and early season corn growth. In: Vyn, T.J. (ed.) Proceedings of the Indiana Certified Crop Adviser Program, December 14-15, Indianapolis, IN. 2004 CDROM.
  6. Kempinski, J., A.V. Stur. 2003. Managing crop root zone ecosystems for prevention of harmful and encouragement of beneficial nematodes. Soil and Tillage Research, Vol. 72, Issue 2, pp 213-221.
  7. Laboski, C. 2006. Does it pay to use nitrification and urease inhibitors? Proc. Wisconsin Fertilizer, Aglime and Pest Management Conf. Univ. Wisc., Madison. Available at http://www.soils.wisc.edu/extension/wfapmc/2006/pap/Laboski1.pdf. (verified 22 August 2008)
  8. Lambert, D. H., D. E. Baker, H. Cole, Jr. 1979. The role of mycorrhizae in the interactions of phosphorus with zinc, copper, and other elements. Soil Sci. Soc. Amer. J., 43:976-980.
  9. Lewandowski, A., Tugel, A. J. 2000. Soil Biology in Rangelands: Key Educational Messages. NRCS-Soil Quality Institute.
  10. Rehm, G., M. Schmitt, J. Lamb, R. Eliason. 2011 revised. Fertilizer recommendations for agronomic crops in Minnesota. University of Minnesota Extension Publication BU-06240-S. Available at http://www.extension.umn.edu/agriculture/nutrient-management/nutrient-lime-guidelines/fertilizer-recommendations-for-agronomic-crops-in-minnesota/.
  11. Tugel, A., A. Lewandowski and D. Happe-von Arb (eds) 2000. Soil biology primer, Rev. ed. Soil and Water Conservation Society. Ankeny, IA. Available at
    www.swcs.org/en/publications/soil_biology_primer/. (verified October 17, 2016)
  12. University of Minnesota, College of Agriculture, Food, and Environmental Sciences. Rhizobium Research Laboratory. 2005. http://grossmanlab.cfans.umn.edu/
  13. USDA-NRCS soil quality index. http://soils.usda.gov/sqi/concepts/soil_biology/nematodes.html
  14. Varsa, E.C., S.A. Ebelhar, P.R. Eberle and Dennis Klockenga. 1996. An Evaluation of Urease Inhibitor Technology as a Nitrogen Management Tool in No-Till Corn Production. Illinois Fertilizer Conference Proceedings. http://frec.cropsci.uiuc.edu/1996/report14/

Revised 2009

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